Terminator

Part:BBa_K5410015:Design

Designed by: Jiewen Wang   Group: iGEM24_GeorgiaState-SWJTU   (2024-10-02)


T7 terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Here are a few design considerations you need to address during the detailed design of gene editing sequences:

1. Off-target effects: When designing the guide RNA (gRNA) or editing tool, it is critical to minimize off-target binding. This requires careful sequence selection to ensure the gRNA binds only to the intended region, avoiding unintended mutations elsewhere in the genome.

2. PAM sequence selection: The Protospacer Adjacent Motif (PAM) is essential for CRISPR-Cas systems to recognize the target DNA sequence. The choice of PAM sequence affects the target range and efficiency of the editing, so identifying compatible and accessible PAM sites is crucial.


Source

The T7 terminator is a sequence that signals the end of transcription by the T7 RNA polymerase. It ensures that transcription stops at the appropriate point, producing correctly sized RNA transcripts. Like the T7 promoter, the T7 terminator is also derived from the T7 bacteriophage genome.

References